Qiagen plasmid maxi kit pdf

Plasmid dna was purified from bacterial cultures using nucleobond xtra midi, nucleobond xtra maxi, and corresponding midiprep and maxiprep kits from qiagen following the protocols for each kit in each case, the maximum input volume specified by the corresponding kit protocol was processed. For qiagen plasmid midi, maxi, mega, and giga kit protocols. Qiagen endofree plasmid maxi kit, 100l to 250ml culture volume, anionexchange technology, transfectiongrade plasmid or cosmid dna purification, manual processing, 150 min. For purification of up to 10 mg transfectiongrade plasmid or cosmid dna. Qiagen plasmid maxi kit, 25 preps, 100 to 500ml culture volume, transfectiongrade. When i use the qiagen plasmid maxi kit to prep the dna for transfection, i cannot get dna pellet. The protocol for qiagen plasmid kits is optimized for use with cultures grown in standard. Qiagen is the leading provider of innovative sample and assay technologies, enabling the isolation. This video shows you how to isolate and purify highquality, endotoxinfree. Qiagen plasmid plus midi kit 25 from qiagen selectscience. Timerun, up to 1mg yield, for the fastest, most convenient purification of up to.

Qiafilter plasmid maxi kit 25, 12263 from qiagen labsave. Qiagen plasmid maxi kit, 25 preps, 100 to 500ml culture volume, transfectiongrade plasmid or cosmid dna purification, anionexchange technology, qiagen plasmid purification handbook 3. Trueblue lysis buffer an optional color indicator is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. I repeated three times and just can not get my dna. The genejet plasmid maxi prep kit provides optimized protocols for plasmid dna purification using low speed up to 5,000. Plasmids and cosmids up to 50 kb in size can be purified using hispeed plasmid kit. Waste disposal at the advanced technology research facility. For the fastest, most convenient purification of up to 10 mg transfectiongrade plasmid dna. Qiagen research kits compatible with the qiavac 24 plus application kit accessories plasmid minipreps qiaprep spin miniprep kits dna cleanup qiaquick. Qiagen plasmid purification handbook sierra bermeja. For purification of up to 10 mg endotoxinfree advanced transfectiongrade plasmid or cosmid dna. Watch this video to learn how to purify plasmid dna using the purelink expi endotoxinfree maxi plasmid purification kit. University of guelph biobar 2020 price list jan 1 through dec. Plasmid dna was purified from bacterial cultures using nucleobond xtra midi, nucleobond xtra maxi, and corresponding midiprep and maxiprep kits from qiagen following the protocols for each kit in.

Qiagen plasmid plus midi kit, 25 preps, 25 to 50ml culture volume. Qiagen plasmid plus midi kit 100, 12945 from qiagen labsave. Endofree plasmid maxi kit 10 from qiagen sample to. Endofree maxi plasmid kitdp117 tianpure midi plasmid kitdp107 tianprep midi plasmid kitdp106 tianprep mini plasmid kitdp103. Genelute hp plasmid maxiprep kit 10 12163 12263 12663 12963. Mix gently by inverting the tube 46 times and incubate at room temperature for exactly 5 min. Cross reference of genelute to qiagen nucleic acid. Endofree maxi plasmid kit dp117 tianpure midi plasmid kit dp107 tianprep midi plasmid kit dp106 tianprep mini plasmid kit dp103. Timerun, up to 1mg yield, ideal transfection of most cell lines, cloning, enzymatic. Qiagen qiafilter plasmid maxi kit, 25 preps, 100 to 250ml culture volume, transfectiongrade plasmid or cosmid dna purification, anionexchange technology, manual processing, 500g yield, 120 min. Qiagen plasmid purification handbook 082003 5 kit contents qiagen plasmid kits qiagen plasmid kit midi 25 midi 100 maxi 10 maxi 25 maxi 100 catalog no. For the qiagen plasmid mini kit protocol microcentrifuge 1. Sample preparation aurum tm plasmid midi and maxi kits.

The dneasy plant maxi kit allows rapid and efficient isolation of highquality dna from a wide variety of plant species and tissue types including the most demanding sources see table selection of plant species processed with dneasy kits. Qiagen plasmid maxi kit 25 from qiagen sample to insight. The qiaprep spin miniprep kit is designed for isolation of up to 20. For fast purification of up to 10 mg transfectiongrade plasmid or cosmid dna. If you have questions about any of the information in the bench. This plasmid facilitates fluorescence assisted sorting of cells and nuclei in addition prepare a mini prep for 1 4 colonies qiaprep spin miniprep kit qiagen.

In addition to the qiagen largeconstruct kit for isolation of up to 50 g ultrapure bac, pac, or p1 dna or up to 200 g ultrapure cosmid dna, qiagen also offers a solution for highthroughput minipreparation of large constructs. These are available online in convenient and compact pdf format at. Plasmid dna purification genomic dna purification cat. No fapde 000maxi 2 preps fapde 003 10 preps fapde 0031. Qiagen plus kit negative control cellsonly control relative luciferase activity purelink fast maxi kit midi kit 1. Favorprep plasmid extraction maxi kit dna isolation kit. The geneaid midi plasmid kit uses prepacked anionexchange resin columns to purify plasmid dna from 50300 ml of cultured bacterial cells. Formulas for qiagen kit buffers genesee scientific. Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol. Do you have any problem with qiagen plasmid maxi kit.

Product information thermo scientific genejet plasmid. When properly stored, all kit components are stable until the expiration date printed on the label. For larger constructs qiagen plasmid kits or the largeconstruct kit are recommended plasmid cosmid copy number plasmid and cosmids vary in copy number, depending on the origin of replication they contain, their size, and the size of insert. Plasmid purification using a qiagen hispeed plasmid maxi kit. Formulas for qiagen kit buffers for long term storage, all buffers should be sterilized by filtration or autoclaving. Following the purification process, a yield of more than 900. For larger constructs qiagen plasmid kits or the largeconstruct kit are recommended plasmidcosmid copy. Trueblue lysis buffer an optional color indicator is. Qiagen plasmid plus midi kit, 25 preps, 25 to 50ml culture volume, transfectiongrade plasmid or cosmid dna purification, advanced silica technology, manual processing, 20 min. Mix gently by inverting the tube 46 times and incubate at room temperature for. Qiagen plasmid plus midi kit, 100 preps, 25 to 50ml culture volume. Add 10 ml of buffer p3, mix gently, incubate on ice for 1520 min. This video shows you how to isolate and purify highquality, endotoxinfree plasmid dna for sensitive applications like primary cell transfection. Qiagen qiafilter plasmid maxi kit, 25 preps, 100 to 250ml culture volume, transfection.

Qiagen plasmid plus midi kit, 100 preps, 25 to 50ml culture volume, transfectiongrade plasmid or cosmid dna purification, advanced silica technology, manual processing, 20 min. Plasmid maxi 25 qiafilter plasmid maxi 25 hispeed plasmid maxi 25 plasmid plus maxi kit 25 na0310. Hispeed plasmid purification handbook kirschner lab. Qiagen 12263 qiafilter plasmid maxi lablifesciencezo.

Solutions that contain ethanol, isopropanol or mops should be sterilized by filtration only. Plasmid or cosmid dna purification using qiagen plasmid mega and giga kits this protocol is designed for preparation of up to 2. All others centrifugation steps should be carried out at room. Hispeed plasmid purification handbook harvard university. Transfections were performed using effectene transfection reagent.

Qiagenplasmidpurificationhandbook 042012 5 qiagen mega5 mega25 giga5 plasmid plasmidkit bufferset catalogno. Midipreps for plasmid dna purificationnucleobond xtra midi. No fapde 000 maxi 2 preps fapde 003 10 preps fapde 0031 20 preps favorprep plasmid extraction maxi kit. Vacuum format protocol overview for complete protocol, consult instruction manual.

Qiagen endofree plasmid maxi kit, 100l to 250ml culture. Timerun, for fast purification of up to 500g transfectiongrade plasmid or cosmid. Qiagen plasmid purification handbook harvard university. Plasmid maxi 10 qiafilter plasmid maxi 10 hispeed plasmid maxi 10 na0300.

Hispeed plasmid midi and maxi kits are developed, designed, and sold for research. Qiagen research kits compatible with the qiavac 24 plus application kit accessories plasmid minipreps qiaprep spin miniprep kits dna cleanup qiaquick pcr purification kits qiaquick gel extraction kits qiaquick nucleotide removal kits minelute pcr purification kits. Plasmid or cosmid dna purification using hispeed plasmid midi. Add 10 ml of buffer p3, mix gently, and incubate on ice for 1520 min.